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Western blot(SDS PAGE) analysis of extracts from A375 cells .Using Methionyl Aminopeptidase 1/MAP 1Rabbit mAb [P07L] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :METAP1,KIAA0094,MAP11_HUMAN,Methionine aminopeptidase 1
UniProtID :P53582
MASS(da) :43,215
MW(kDa) :43 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Methionyl Aminopeptidase 1/MAP 1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A375 cells
Function : Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Required for normal progression through the cell cycle..
Subcellular locationi :Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.