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Mimitin Rabbit mAb [8VY1]Cat NO.: A36337

Western blot(SDS PAGE) analysis of extracts from HepG2 cells .Using MimitinRabbit mAb [8VY1] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :NDUFAF2,NDUFA12L,NDUF2_HUMAN,NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2

UniProtID :Q8N183

MASS(da) :19,856

MW(kDa) :20 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Mimitin

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HepG2 cells 

Function : Acts as a molecular chaperone for mitochondrial complex I assembly (PubMed:16200211, PubMed:19384974). Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (PubMed:16200211, PubMed:27626371)..

Tissue specificity :Highly expressed in ESCC cells. Also expressed in heart, skeletal muscle, liver, and in fibroblasts..

Subcellular locationi :Mitochondrion.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.