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Western blot(SDS PAGE) analysis of extracts from HepG2 cells .Using MimitinRabbit mAb [8VY1] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :NDUFAF2,NDUFA12L,NDUF2_HUMAN,NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2
UniProtID :Q8N183
MASS(da) :19,856
MW(kDa) :20 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Mimitin
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Acts as a molecular chaperone for mitochondrial complex I assembly (PubMed:16200211, PubMed:19384974). Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (PubMed:16200211, PubMed:27626371)..
Tissue specificity :Highly expressed in ESCC cells. Also expressed in heart, skeletal muscle, liver, and in fibroblasts..
Subcellular locationi :Mitochondrion.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.