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PRPF4 Rabbit mAb [0W78]Cat NO.: A71659

Western blot(SDS PAGE) analysis of extracts from SW480 cells.Using PRPF4Rabbit mAb [0W78] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :PRPF4,PRP4,PRP4_HUMAN,U4/U6 small nuclear ribonucleoprotein Prp4

UniProtID :O43172

MASS(da) :58,449

MW(kDa) :58 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Rat,Mouse

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human PRPF4

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :SW480 cells

Function : Plays role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex)..

Subcellular locationi :Nucleus. Nucleus speckle.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.