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Western blot(SDS PAGE) analysis of extracts from SW480 cells.Using PRPF4Rabbit mAb [0W78] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :PRPF4,PRP4,PRP4_HUMAN,U4/U6 small nuclear ribonucleoprotein Prp4
UniProtID :O43172
MASS(da) :58,449
MW(kDa) :58 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Rat,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human PRPF4
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :SW480 cells
Function : Plays role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex)..
Subcellular locationi :Nucleus. Nucleus speckle.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.