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TPN Rabbit mAb [20V9]Cat NO.: A67662

Western blot(SDS PAGE) analysis of extracts from HeLa cells treated with 100 ng/ml IFN gamma for 16 hours.Using TPNRabbit mAb [20V9] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :TAPBP,NGS17,TAPA,TPSN_HUMAN,Tapasin

UniProtID :O15533

MASS(da) :47,571

MW(kDa) :48 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human TPN

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells treated with 100 ng/ml IFN gamma for 16 hours

Function : Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading)..

Tissue specificity :Neutrophils, mostly in fully differentiated cells.

Subcellular locationi :Endoplasmic reticulum membrane,Single-pass type I membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.