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Western blot(SDS PAGE) analysis of extracts from HT1080 CELLS.Using Cathepsin BRabbit mAb [WN71] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CTSB,CPSB,CATB_HUMAN,Cathepsin B [Cleaved into: Cathepsin B light chain,Cathepsin B heavy chain]
UniProtID :P07858
MASS(da) :37,822
MW(kDa) :37 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Cathepsin B
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HT1080 CELLS
Function : Thiol protease which is believed to participate in intracellular degradation and turnover of proteins (PubMed:12220505). Cleaves matrix extracellular phosphoglycoprotein MEPE (PubMed:12220505). Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen (By similarity). Has also been implicated in tumor invasion and metastasis (PubMed:3972105)..
Tissue specificity :Expressed in the stratum spinosum of the epidermis. Weak expression is detected in the stratum granulosum..
Subcellular locationi :Lysosome. Melanosome. Secreted, extracellular space. Apical cell membrane,Peripheral membrane protein,Extracellular side.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.