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Cathepsin B Rabbit mAb [WN71]Cat NO.: A31307

Western blot(SDS PAGE) analysis of extracts from HT1080 CELLS.Using Cathepsin BRabbit mAb [WN71] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :CTSB,CPSB,CATB_HUMAN,Cathepsin B [Cleaved into: Cathepsin B light chain,Cathepsin B heavy chain]

UniProtID :P07858

MASS(da) :37,822

MW(kDa) :37 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Cathepsin B

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HT1080 CELLS

Function : Thiol protease which is believed to participate in intracellular degradation and turnover of proteins (PubMed:12220505). Cleaves matrix extracellular phosphoglycoprotein MEPE (PubMed:12220505). Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen (By similarity). Has also been implicated in tumor invasion and metastasis (PubMed:3972105)..

Tissue specificity :Expressed in the stratum spinosum of the epidermis. Weak expression is detected in the stratum granulosum..

Subcellular locationi :Lysosome. Melanosome. Secreted, extracellular space. Apical cell membrane,Peripheral membrane protein,Extracellular side.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.