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Cytokeratin 4 Rabbit mAb [4425]Cat NO.: A97195

Western blot(SDS PAGE) analysis of extracts from A431 cells .Using Cytokeratin 4Rabbit mAb [4425] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :KRT4,CYK4,K2C4_HUMAN,Keratin, type II cytoskeletal 4

UniProtID :P19013

MASS(da) :56,144

MW(kDa) :59 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Cytokeratin 4

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :A431 cells 

Tissue specificity :Detected in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa (at protein level). Expressed widely in the exocervix and esophageal epithelium, with lowest levels detected in the basal cell layer..

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.