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Western blot(SDS PAGE) analysis of extracts from A431 cells .Using NDUFB7Rabbit mAb [D8KN] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :NDUFB7,NDUB7_HUMAN,NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7
UniProtID :P17568
MASS(da) :16,402
MW(kDa) :16 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human NDUFB7
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A431 cells
Function : Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone..
Subcellular locationi :Mitochondrion inner membrane,Peripheral membrane protein. Mitochondrion intermembrane space.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.