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Western blot(SDS PAGE) analysis of extracts from Human liver.Using TDO2/TDORabbit mAb [D7R2] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TDO2,TDO,T23O_HUMAN,Tryptophan 2,3-dioxygenase
UniProtID :P48775
MASS(da) :47,872
MW(kDa) :43 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human TDO2/TDO
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected : Human liver
Function : Heme-dependent dioxygenase that catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring and converts L-tryptophan to N-formyl-L-kynurenine. Catalyzes the oxidative cleavage of the indole moiety..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.