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Claudin 4 Rabbit mAb [8EJ0]Cat NO.: A88230

Western blot(SDS PAGE) analysis of extracts from SW480 cells.Using Claudin 4Rabbit mAb [8EJ0] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :CLDN4,CPER,CPETR1,WBSCR8,CLD4_HUMAN,Claudin-4

UniProtID :O14493

MASS(da) :22,077

MW(kDa) :18 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Claudin 4

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :SW480 cells

Function : Channel-forming tight junction protein that mediates paracellular chloride transport in the kidney. Plays a critical role in the paracellular reabsorption of filtered chloride in the kidney collecting ducts. Claudins play a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity..

Subcellular locationi :Cell junction, tight junction. Cell membrane,Multi-pass membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.