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Western blot(SDS PAGE) analysis of extracts from HEK293 cells .Using HIRA/HIRRabbit mAb [4EHA] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :HIRA,DGCR1,HIR,TUPLE1,HIRA_HUMAN,Protein HIRA
UniProtID :P54198
MASS(da) :111,835
MW(kDa) :112 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human HIRA/HIR
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HEK293 cells
Function : Cooperates with ASF1A to promote replication-independent chromatin assembly. Required for the periodic repression of histone gene transcription during the cell cycle. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit..
Tissue specificity :Expressed at high levels in kidney, pancreas and skeletal muscle and at lower levels in brain, heart, liver, lung, and placenta..
Subcellular locationi :Nucleus. Nucleus, PML body.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.