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Western blot(SDS PAGE) analysis of extracts from Jurkat cells .Using BidRabbit mAb [16NH] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :BID,BID_HUMAN,BH3-interacting domain death agonist [Cleaved into: BH3-interacting domain death agonist p15 ,BH3-interacting domain death agonist p13 ,BH3-interacting domain death agonist p11 ]
UniProtID :P55957
MASS(da) :21,995
MW(kDa) :22 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Bid
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Jurkat cells
Function : Induces caspases and apoptosis (PubMed:14583606). Counters the protective effect of BCL2 (By similarity).., [BH3-interacting domain death agonist p15]: Induces caspase activation and apoptosis (PubMed:15661737, PubMed:32029622). Allows the release of cytochrome c (PubMed:32029622).., [Isoform 1]: Induces ICE-like proteases and apoptosis.., [Isoform 2]: Induces ICE-like proteases and apoptosis.., [Isoform 3]: Does not induce apoptosis.., [Isoform 4]: Induces ICE-like proteases and apoptosis..
Tissue specificity :[Isoform 2]: Expressed in spleen, pancreas and placenta (at protein level)..,[Isoform 3]: Expressed in lung, pancreas and spleen (at protein level)..,[Isoform 4]: Expressed in lung and pancreas (at protein level)..
Subcellular locationi :Cytoplasm. Mitochondrion membrane. Mitochondrion outer membrane.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.