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Western blot(SDS PAGE) analysis of extracts from K562 cells .Using ARF4Rabbit mAb [H021] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ARF4,ARF2,ARF4_HUMAN,ADP-ribosylation factor 4
UniProtID :P18085
MASS(da) :20,511
MW(kDa) :25 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human ARF4
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :K562 cells
Function : GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking,may modulate vesicle budding and uncoating within the Golgi apparatus.
Subcellular locationi :Golgi apparatus. Membrane,Lipid-anchor.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.