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Western blot(SDS PAGE) analysis of extracts from HEK293 cells .Using CAD/BM1Rabbit mAb [D36J] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CAD,PYR1_HUMAN,CAD protein [Includes: Glutamine-dependent carbamoyl-phosphate synthase ,Aspartate carbamoyltransferase ,Dihydroorotase ]
UniProtID :P27708
MASS(da) :242,984
MW(kDa) :250 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human CAD/BM1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HEK293 cells
Function : This protein is a 'fusion' protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase)..
Subcellular locationi :Cytoplasm. Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.