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Endostatin/COL18A1 Rabbit mAb [Q540]Cat NO.: A25773

Western blot(SDS PAGE) analysis of extracts from HepG2 cells .Using Endostatin/COL18A1Rabbit mAb [Q540] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :COL18A1,COIA1_HUMAN,Collagen alpha-1 chain [Cleaved into: Endostatin,Non-collagenous domain 1 ]

UniProtID :P39060

MASS(da) :178,188

MW(kDa) :178 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Endostatin/COL18A1

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HepG2 cells 

Function : Probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.., [Non-collagenous domain 1]: May regulate extracellular matrix-dependent motility and morphogenesis of endothelial and non-endothelial cells,the function requires homotrimerization and implicates MAPK signaling.., [Endostatin]: Potently inhibits endothelial cell proliferation and angiogenesis (PubMed:9459295). May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling (By similarity). Inhibits VEGFA-induced endothelial cell proliferation and migration. Seems to inhibit VEGFA-mediated signaling by blocking the interaction of VEGFA to its receptor KDR/VEGFR2. Modulates endothelial cell migration in an integrin-dependent manner implicating integrin ITGA5:ITGB1 and to a lesser extent ITGAV:ITGB3 and ITGAV:ITGB5 (By similarity). May negatively regulate the activity of homotrimeric non-collagenous domain 1 (PubMed:11257123)..

Tissue specificity :Present in multiple organs with highest levels in liver, lung and kidney.

Subcellular locationi :Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, extracellular matrix, basement membrane.,[Non-collagenous domain 1]: Secreted, extracellular space, extracellular matrix, basement membrane. Secreted.,[Endostatin]: Secreted. Secreted, extracellular space, extracellular matrix, basement membrane.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.