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Western blot(SDS PAGE) analysis of extracts from HepG2 cells .Using Endostatin/COL18A1Rabbit mAb [Q540] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :COL18A1,COIA1_HUMAN,Collagen alpha-1 chain [Cleaved into: Endostatin,Non-collagenous domain 1 ]
UniProtID :P39060
MASS(da) :178,188
MW(kDa) :178 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Endostatin/COL18A1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.., [Non-collagenous domain 1]: May regulate extracellular matrix-dependent motility and morphogenesis of endothelial and non-endothelial cells,the function requires homotrimerization and implicates MAPK signaling.., [Endostatin]: Potently inhibits endothelial cell proliferation and angiogenesis (PubMed:9459295). May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling (By similarity). Inhibits VEGFA-induced endothelial cell proliferation and migration. Seems to inhibit VEGFA-mediated signaling by blocking the interaction of VEGFA to its receptor KDR/VEGFR2. Modulates endothelial cell migration in an integrin-dependent manner implicating integrin ITGA5:ITGB1 and to a lesser extent ITGAV:ITGB3 and ITGAV:ITGB5 (By similarity). May negatively regulate the activity of homotrimeric non-collagenous domain 1 (PubMed:11257123)..
Tissue specificity :Present in multiple organs with highest levels in liver, lung and kidney.
Subcellular locationi :Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, extracellular matrix, basement membrane.,[Non-collagenous domain 1]: Secreted, extracellular space, extracellular matrix, basement membrane. Secreted.,[Endostatin]: Secreted. Secreted, extracellular space, extracellular matrix, basement membrane.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.