* 产品详情- */>
Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using IMPDH2Rabbit mAb [JR3L] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :IMPDH2,IMPD2,IMDH2_HUMAN,Inosine-5'-monophosphate dehydrogenase 2
UniProtID :P12268
MASS(da) :55,805
MW(kDa) :56 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human IMPDH2
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth (PubMed:7903306, PubMed:7763314). Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism (PubMed:14766016). It may also have a role in the development of malignancy and the growth progression of some tumors..
Tissue specificity :IMPDH1 is the main species in normal leukocytes and IMPDH2 predominates over IMPDH1 in the tumor.
Subcellular locationi :Cytoplasm. Nucleus. Cytoplasm, cytosol.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.