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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using TOMM22/TOM22Rabbit mAb [P966] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TOMM22,TOM22,TOM22_HUMAN,Mitochondrial import receptor subunit TOM22 homolog
UniProtID :Q9NS69
MASS(da) :15,522
MW(kDa) : 16kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human TOMM22/TOM22
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Central receptor component of the translocase of the outer membrane of mitochondria (TOM complex) responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with the peripheral receptor TOM20 functions as the transit peptide receptor and facilitates the movement of preproteins into the translocation pore (PubMed:10982837). Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases (By similarity)..
Tissue specificity :Ubiquitous.
Subcellular locationi :Mitochondrion outer membrane,Single-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.