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Western blot(SDS PAGE) analysis of extracts from Ramos cells.Using U2AF35/U2AF1Rabbit mAb [Y4GD] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :U2AF1,U2AF35,U2AFBP,FP793,U2AF1_HUMAN,Splicing factor U2AF 35 kDa subunit
UniProtID :Q01081
MASS(da) :27,872
MW(kDa) :35 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human U2AF35/U2AF1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Ramos cells
Function : Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron..
Subcellular locationi :Nucleus. Nucleus speckle.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.