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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using ABCF1Rabbit mAb [LCR2] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ABCF1,ABC50,ABCF1_HUMAN,ATP-binding cassette sub-family F member 1
UniProtID :Q8NE71
MASS(da) :95,926
MW(kDa) :95 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human ABCF1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Isoform 2 is required for efficient Cap- and IRES-mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis..
Tissue specificity :Ubiquitous..
Subcellular locationi :[Isoform 2]: Cytoplasm. Nucleus, nucleoplasm. Nucleus envelope.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.