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Western blot(SDS PAGE) analysis of extracts from SKBR-3 .Using HDGFRabbit mAb [APTC] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :HDGF,HMG1L2,HDGF_HUMAN,Hepatoma-derived growth factor
UniProtID :P51858
MASS(da) :26,788
MW(kDa) :26 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human HDGF
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :SKBR-3
Function : [Isoform 1]: Acts as a transcriptional repressor (PubMed:17974029). Has mitogenic activity for fibroblasts (PubMed:11751870, PubMed:26845719). Heparin-binding protein (PubMed:15491618).., [Isoform 2]: Does not have mitogenic activity for fibroblasts (PubMed:26845719). Does not bind heparin (PubMed:26845719).., [Isoform 3]: Has mitogenic activity for fibroblasts (PubMed:26845719). Heparin-binding protein (PubMed:26845719)..
Tissue specificity :Ubiquitous..
Subcellular locationi :[Isoform 1]: Nucleus. Cytoplasm. Secreted, extracellular exosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.