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Western blot(SDS PAGE) analysis of extracts from HT1376.Using MUC2Rabbit mAb [55T3] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MUC2,SMUC,MUC2_HUMAN,Mucin-2
UniProtID :Q02817
MASS(da) :540,300
MW(kDa) :130 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human MUC2
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HT1376
Function : Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer..
Tissue specificity :Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder.
Subcellular locationi :Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.