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Western blot(SDS PAGE) analysis of extracts from Human fetal kidney .Using PPA2Rabbit mAb [9818] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :PPA2,HSPC124,IPYR2_HUMAN,Inorganic pyrophosphatase 2, mitochondrial
UniProtID :Q9H2U2
MASS(da) :37,920
MW(kDa) :35 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human PPA2
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Human fetal kidney
Function : Hydrolyzes inorganic pyrophosphate (PubMed:27523597). This activity is essential for correct regulation of mitochondrial membrane potential, and mitochondrial organization and function (PubMed:27523598)..
Tissue specificity :Detected in brain, gastric carcinoma, lung, ovary, skeletal muscle, umbilical cord blood and a cell line derived from kidney proximal tubule epithelium..
Subcellular locationi :Mitochondrion.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.