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Tat-SF1 Rabbit mAb [GD1L]Cat NO.: A15825

Western blot(SDS PAGE) analysis of extracts from HeLa cells .Using Tat-SF1Rabbit mAb [GD1L] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :HTATSF1,HTSF1_HUMAN,HIV Tat-specific factor 1

UniProtID :O43719

MASS(da) :85,853

MW(kDa) :140 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Tat-SF1

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells 

Function : Functions as a general transcription factor playing a role in the process of transcriptional elongation. May mediate the reciprocal stimulatory effect of splicing on transcriptional elongation. In case of infection by HIV-1, it is up-regulated by the HIV-1 proteins NEF and gp120, acts as a cofactor required for the Tat-enhanced transcription of the virus..

Tissue specificity :Widely expressed..

Subcellular locationi :Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.