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TSPEAR Rabbit mAb [20J7]Cat NO.: A19789

Western blot(SDS PAGE) analysis of extracts from Raw264.7 cells .Using TSPEARRabbit mAb [20J7] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :TSPEAR,C21orf29,TSEAR_HUMAN,Thrombospondin-type laminin G domain and EAR repeat-containing protein

UniProtID :Q8WU66

MASS(da) :74,924

MW(kDa) :80 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human TSPEAR

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Raw264.7 cells 

Function : Plays a critical role in tooth and hair follicle morphogenesis through regulation of the Notch signaling pathway (PubMed:27736875). May play a role in development or function of the auditory system (PubMed:22678063)..

Subcellular locationi :Secreted. Cell surface. Cell projection, stereocilium.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.