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Western blot(SDS PAGE) analysis of extracts from Raw264.7 cells .Using TSPEARRabbit mAb [20J7] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TSPEAR,C21orf29,TSEAR_HUMAN,Thrombospondin-type laminin G domain and EAR repeat-containing protein
UniProtID :Q8WU66
MASS(da) :74,924
MW(kDa) :80 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human TSPEAR
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Raw264.7 cells
Function : Plays a critical role in tooth and hair follicle morphogenesis through regulation of the Notch signaling pathway (PubMed:27736875). May play a role in development or function of the auditory system (PubMed:22678063)..
Subcellular locationi :Secreted. Cell surface. Cell projection, stereocilium.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.