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P-14-3-3 ζ/δ (S58)/η (S59)/γ (S59)/β/α (S60) Rabbit mAb [980N]Cat NO.: A98053

Western blot(SDS PAGE) analysis of extracts from HeLa cells treated with IBMX (50 uM) and Forskolin (30 min).Using P-14-3-3 ζ/δ (S58)/η (S59)/γ (S59)/β/α (S60) Rabbit mAb [980N] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :YWHAG,1433G_HUMAN,14-3-3 protein gamma [Cleaved into: 14-3-3 protein gamma, N-terminally processed]

UniProtID :P61981

MASS(da) :28,303

MW(kDa) :28 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human Phospho-14-3-3 ζ/δ (Ser58)/η (Ser59)/γ (Ser59)/β/α (Ser60)

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells treated with IBMX (50 uM) and Forskolin (30 min)

Function : Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner..

Tissue specificity :Highly expressed in brain, skeletal muscle, and heart..

Subcellular locationi :Cytoplasm.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.