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Western blot(SDS PAGE) analysis of extracts from Mouse brain .Using Metabotropic Glutamate Receptor 3/MGLUR3Rabbit mAb [U5UX] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :GRM3,GPRC1C,MGLUR3,GRM3_HUMAN,Metabotropic glutamate receptor 3
UniProtID :Q14832
MASS(da) :98,879
MW(kDa) :220 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Metabotropic Glutamate Receptor 3/MGLUR3
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Mouse brain
Function : G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity..
Tissue specificity :Detected in brain cortex, thalamus, subthalamic nucleus, substantia nigra, hypothalamus, hippocampus, corpus callosum, caudate nucleus and amygdala..
Subcellular locationi :Cell membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.