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Ras Rabbit mAb [YWPO]Cat NO.: A48841

Western blot(SDS PAGE) analysis of extracts from Mouse brain.Using RasRabbit mAb [YWPO] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :HRAS,HRAS1,RASH_HUMAN,GTPase HRas [Cleaved into: GTPase HRas, N-terminally processed]

UniProtID :P01112

MASS(da) :21,298

MW(kDa) :21 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Ras

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Mouse brain

Function : Involved in the activation of Ras protein signal transduction (PubMed:22821884). Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed:12740440, PubMed:14500341, PubMed:9020151)..

Tissue specificity :Widely expressed..

Subcellular locationi :Cell membrane,Lipid-anchor,Cytoplasmic side. Golgi apparatus. Golgi apparatus membrane,Lipid-anchor.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.