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Western blot(SDS PAGE) analysis of extracts from 293T cells.Using eIF1ARabbit mAb [U04V] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :EIF1AX,EIF1A,EIF4C,IF1AX_HUMAN,Eukaryotic translation initiation factor 1A, X-chromosomal
UniProtID :P47813
MASS(da) :16,460
MW(kDa) :16 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human eIF1A
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :293T cells
Function : Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.