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Western blot(SDS PAGE) analysis of extracts from U937 cells.Using FLI1Rabbit mAb [KSRV] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :FLI1,FLI1_HUMAN,Friend leukemia integration 1 transcription factor
UniProtID :Q01543
MASS(da) :50,982
MW(kDa) :51 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human FLI1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :U937 cells
Function : Sequence-specific transcriptional activator (PubMed:24100448, PubMed:26316623, PubMed:28255014). Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'..
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.