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MAP1LC3A Rabbit mAb [NAIF]Cat NO.: A52203

Western blot(SDS PAGE) analysis of extracts from HeLa cells .Using MAP1LC3ARabbit mAb [NAIF] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :MAP1LC3A,MLP3A_HUMAN,Microtubule-associated proteins 1A/1B light chain 3A

UniProtID :Q9H492

MASS(da) :14,272

MW(kDa) :16 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human MAP1LC3A

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells 

Function : Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed:20713600, PubMed:24290141). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:20713600). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006538, PubMed:31006537)..

Tissue specificity :Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes..

Subcellular locationi :Cytoplasmic vesicle, autophagosome membrane,Lipid-anchor. Endomembrane system,Lipid-anchor. Cytoplasm, cytoskeleton.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.