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Western blot(SDS PAGE) analysis of extracts from HeLa cells .Using MAP1LC3ARabbit mAb [NAIF] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MAP1LC3A,MLP3A_HUMAN,Microtubule-associated proteins 1A/1B light chain 3A
UniProtID :Q9H492
MASS(da) :14,272
MW(kDa) :16 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human MAP1LC3A
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed:20713600, PubMed:24290141). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:20713600). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006538, PubMed:31006537)..
Tissue specificity :Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes..
Subcellular locationi :Cytoplasmic vesicle, autophagosome membrane,Lipid-anchor. Endomembrane system,Lipid-anchor. Cytoplasm, cytoskeleton.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.