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Western blot(SDS PAGE) analysis of extracts from Mouse cerebellum .Using Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3Rabbit mAb [0P73] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :PLCB3,PLCB3_HUMAN,1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3
UniProtID :Q01970,Q9NQ66
MASS(da) :138,799
MW(kDa) :150 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Mouse cerebellum
Function : The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes..
Subcellular locationi :Cytoplasm. Membrane. Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.