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Western blot(SDS PAGE) analysis of extracts from HEK-293T cells transfected with POMC .Using POMCRabbit mAb [4ADC] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :POMC,COLI_HUMAN,Pro-opiomelanocortin [Cleaved into: NPP,Melanotropin gamma ,Potential peptide,Corticotropin ,Melanocyte-stimulating hormone alpha ,Corticotropin-like intermediary peptide ,Lipotropin beta ,Lipotropin gamma ,Melanocyte-stimulating hormo
UniProtID :P01189
MASS(da) :29,424
MW(kDa) :29 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human POMC
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HEK-293T cells transfected with POMC
Function : [Corticotropin]: Stimulates the adrenal glands to release cortisol., [Melanocyte-stimulating hormone alpha]: Anorexigenic peptide. Increases the pigmentation of skin by increasing melanin production in melanocytes., [Melanocyte-stimulating hormone beta]: Increases the pigmentation of skin by increasing melanin production in melanocytes., [Beta-endorphin]: Endogenous orexigenic opiate., [Met-enkephalin]: Endogenous opiate.
Tissue specificity :ACTH and MSH are produced by the pituitary gland.
Subcellular locationi :Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.