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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using Radixin/RDXRabbit mAb [6QDH] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :RDX,RADI_HUMAN,Radixin
UniProtID :P35241
MASS(da) :68,564
MW(kDa) :80 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Radixin/RDX
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Probably plays a crucial role in the binding of the barbed end of actin filaments to the plasma membrane.
Subcellular locationi :Cell membrane,Peripheral membrane protein,Cytoplasmic side. Cytoplasm, cytoskeleton. Cleavage furrow. Cell projection, microvillus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.