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Western blot(SDS PAGE) analysis of extracts from rat testis .Using REC8Rabbit mAb [60X4] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :REC8,REC8L1,REC8_HUMAN,Meiotic recombination protein REC8 homolog
UniProtID :O95072
MASS(da) :62,614
MW(kDa) :62 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human REC8
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :rat testis
Function : Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II (By similarity)..
Tissue specificity :Expressed in testis and thymus. Expressed in the B-cell lines WI-L2-NS and Namalwa (at protein level)..
Subcellular locationi :Nucleus. Chromosome. Chromosome, centromere.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.