RHEB Rabbit mAb [4TFT]Cat NO.: A88187

Protein names ：RHEB,RHEB2,RHEB_HUMAN,GTP-binding protein Rheb

UniProtID ：Q15382

MASS(da) ：20,497

MW(kDa) ：16 kDa

Form ：Liquid

Purification ：Protein A purification

Host ：Rabbit

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• 免疫组化(IHC)1:100
• 免疫荧光(ICC/IF) 1:100
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human RHEB

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：SH-SY5Y cells 

Function ： Activates the protein kinase activity of mTORC1, and thereby plays a role in the regulation of apoptosis. Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Has low intrinsic GTPase activity..

Tissue specificity ：Ubiquitous. Highest levels observed in skeletal and cardiac muscle..

Subcellular locationi ：Endomembrane system,Lipid-anchor,Cytoplasmic side. Golgi apparatus membrane,Lipid-anchor,Cytoplasmic side. Cytoplasm, cytosol. Endoplasmic reticulum membrane,Lipid-anchor,Cytoplasmic side.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.