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Western blot(SDS PAGE) analysis of extracts from K562 cells.Using TIM44Rabbit mAb [7QUW] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TIMM44,MIMT44,TIM44,TIM44_HUMAN,Mitochondrial import inner membrane translocase subunit TIM44
UniProtID :O43615
MASS(da) :51,356
MW(kDa) :45 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human TIM44
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :K562 cells
Function : Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner (By similarity). Recruits mitochondrial HSP70 to drive protein translocation into the matrix using ATP as an energy source (By similarity)..
Subcellular locationi :Mitochondrion inner membrane,Peripheral membrane protein,Matrix side. Mitochondrion matrix.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.