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Western blot(SDS PAGE) analysis of extracts from L6.Using VPS34Rabbit mAb [A91D] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :PIK3C3,VPS34,PK3C3_HUMAN,Phosphatidylinositol 3-kinase catalytic subunit type 3
UniProtID :Q8NEB9
MASS(da) :101,549
MW(kDa) :100 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human VPS34
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :L6
Function : Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate,different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis (PubMed:14617358, PubMed:7628435, PubMed:33637724). As part of PI3KC3-C1, promotes endoplasmic reticulum membrane curvature formation prior to vesicle budding (PubMed:32690950). Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20208530, PubMed:20643123). Involved in the transport of lysosomal enzyme precursors to lysosomes (By similarity). Required for transport from early to late endosomes (By similarity).., (Microbial infection) Kinase activity is required for SARS coronavirus-2/SARS-CoV-2 replication..
Tissue specificity :Ubiquitously expressed, with a highest expression in skeletal muscle..
Subcellular locationi :Midbody. Late endosome. Cytoplasmic vesicle, autophagosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.