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Western blot(SDS PAGE) analysis of extracts from human fetal heart .Using ATP6V1ARabbit mAb [Y452] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ATP6V1A,ATP6A1,ATP6V1A1,VPP2,VATA_HUMAN,V-type proton ATPase catalytic subunit A
UniProtID :P38606
MASS(da) :68,304
MW(kDa) :68 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human ATP6V1A
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :human fetal heart
Function : Catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (PubMed:8463241). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (PubMed:32001091). In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation (PubMed:28296633). May play a role in neurite development and synaptic connectivity (PubMed:29668857).., (Microbial infection) Plays an important role in virion uncoating during Rabies virus replication after membrane fusion. Specifically, participates in the dissociation of incoming viral matrix M proteins uncoating through direct interaction..
Tissue specificity :High expression in the skin..
Subcellular locationi :Cytoplasm. Cytoplasm, cytosol. Cytoplasmic vesicle, secretory vesicle. Cytoplasmic vesicle, clathrin-coated vesicle membrane,Peripheral membrane protein. Lysosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.