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Western blot(SDS PAGE) analysis of extracts from Jurkat cells.Using Sumo 2 + Sumo 3Rabbit mAb [R0I2] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SUMO3,SMT3A,SMT3H1,SUMO3_HUMAN,Small ubiquitin-related modifier 3
UniProtID :P55854,P61956
MASS(da) :10,871
MW(kDa) :16 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Sumo 2 + Sumo 3
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Jurkat cells
Function : Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4 (PubMed:11451954, PubMed:18538659, PubMed:21965678). Plays a role in the regulation of sumoylation status of SETX (PubMed:24105744)..
Tissue specificity :Expressed predominantly in liver..
Subcellular locationi :Cytoplasm. Nucleus. Nucleus, PML body.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.