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Western blot(SDS PAGE) analysis of extracts from Jurkat cells .Using UCHL5IPRabbit mAb [ULF3] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :HAUS7,UCHL5IP,UIP1,HAUS7_HUMAN,HAUS augmin-like complex subunit 7
UniProtID :Q99871
MASS(da) :39,794
MW(kDa) :41 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human UCHL5IP
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Jurkat cells
Function : Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex..
Tissue specificity :Detected in spleen, thymus, testis, ovary, small intestine and colon, with highest levels of expression in testis and ovary..
Subcellular locationi :Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.