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AGO4 Rabbit mAb [3P5D]Cat NO.: A79856

Western blot(SDS PAGE) analysis of extracts from  HEK-293T cells.Using AGO4Rabbit mAb [3P5D] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :AGO4,EIF2C4,KIAA1567,AGO4_HUMAN,Protein argonaute-4

UniProtID :Q9HCK5

MASS(da) :97,097

MW(kDa) :97 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human AGO4

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected : HEK-293T cells

Function : Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for RNA-directed transcription and replication of the human hapatitis delta virus (HDV)..

Subcellular locationi :Cytoplasm, P-body.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.