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ALDH4A1/P5CDH Rabbit mAb [P224]Cat NO.: A25867

Western blot(SDS PAGE) analysis of extracts from K562 cells .Using ALDH4A1/P5CDHRabbit mAb [P224] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :ALDH4A1,ALDH4,P5CDH,AL4A1_HUMAN,Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial

UniProtID :P30038

MASS(da) :61,719

MW(kDa) :62 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human ALDH4A1/P5CDH

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :K562 cells 

Function : Irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes..

Tissue specificity :Highest expression is found in liver followed by skeletal muscle, kidney, heart, brain, placenta, lung and pancreas.

Subcellular locationi :Mitochondrion matrix.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.