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Western blot(SDS PAGE) analysis of extracts from K562 cells .Using ALDH4A1/P5CDHRabbit mAb [P224] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ALDH4A1,ALDH4,P5CDH,AL4A1_HUMAN,Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial
UniProtID :P30038
MASS(da) :61,719
MW(kDa) :62 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human ALDH4A1/P5CDH
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :K562 cells
Function : Irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes..
Tissue specificity :Highest expression is found in liver followed by skeletal muscle, kidney, heart, brain, placenta, lung and pancreas.
Subcellular locationi :Mitochondrion matrix.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.