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Western blot(SDS PAGE) analysis of extracts from BXPC-3 CELLS.Using GCATRabbit mAb [J122] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :GCAT,KBL,KBL_HUMAN,2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial
UniProtID :O75600
MASS(da) :45,285
MW(kDa) :45 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human GCAT
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :BXPC-3 CELLS
Function : Pyridoxal phosphate (PLP) dependent enzyme, which catalyzes the cleavage of 2-amino-3-oxobutanoate to glycine and acetyl-CoA..
Tissue specificity :Strongly expressed in heart, brain, liver and pancreas. Also found in lung..
Subcellular locationi :Mitochondrion. Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.