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GCAT Rabbit mAb [J122]Cat NO.: A63311

Western blot(SDS PAGE) analysis of extracts from BXPC-3 CELLS.Using GCATRabbit mAb [J122] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :GCAT,KBL,KBL_HUMAN,2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial

UniProtID :O75600

MASS(da) :45,285

MW(kDa) :45 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human GCAT

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :BXPC-3 CELLS

Function : Pyridoxal phosphate (PLP) dependent enzyme, which catalyzes the cleavage of 2-amino-3-oxobutanoate to glycine and acetyl-CoA..

Tissue specificity :Strongly expressed in heart, brain, liver and pancreas. Also found in lung..

Subcellular locationi :Mitochondrion. Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.