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Western blot(SDS PAGE) analysis of extracts from A673 .Using GPCR GPR64Rabbit mAb [XIFL] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ADGRG2,GPR64,HE6,TM7LN2,AGRG2_HUMAN,Adhesion G-protein coupled receptor G2
UniProtID :Q8IZP9
MASS(da) :111,593
MW(kDa) :105,130,180 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human GPCR GPR64
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A673
Function : Orphan receptor. Could be involved in a signal transduction pathway controlling epididymal function and male fertility. May regulate fluid exchange within epididymis..
Tissue specificity :Epididymis-specific expression (at protein level). Both subunits are associated with apical membranes of efferent ductule and proximal epididymal duct epithelia. Mainly expressed in the nonciliated principal cells of the proximal excurrent ducts. Specifically over-expressed in Ewing sarcomas but also up-regulated in a number of carcinomas derived from prostate, kidney or lung..
Subcellular locationi :Apical cell membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.