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Western blot(SDS PAGE) analysis of extracts from HepG2 cells .Using Lipin 1Rabbit mAb [YEN8] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :LPIN1,KIAA0188,LPIN1_HUMAN,Phosphatidate phosphatase LPIN1
UniProtID :Q14693
MASS(da) :98,664
MW(kDa) :99 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Lipin 1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis and therefore controls the metabolism of fatty acids at different levels (PubMed:20231281, PubMed:29765047). Is involved in adipocyte differentiation (By similarity). Acts also as nuclear transcriptional coactivator for PPARGC1A/PPARA regulatory pathway to modulate lipid metabolism gene expression (By similarity). Recruited at the mitochondrion outer membrane and is involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol (By similarity)..
Tissue specificity :Specifically expressed in skeletal muscle. Also abundant in adipose tissue. Lower levels in some portions of the digestive tract..
Subcellular locationi :Cytoplasm, cytosol. Endoplasmic reticulum membrane. Nucleus membrane.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.