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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using MGST3Rabbit mAb [5SC5] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MGST3,MGST3_HUMAN,Microsomal glutathione S-transferase 3
UniProtID :O14880
MASS(da) :16,516
MW(kDa) :17 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human MGST3
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Catalyzes oxydation of hydroxy-fatty acids (PubMed:9278457). Also catalyzes the conjugation of a reduced glutathione to leukotriene A4 in vitro (PubMed:9278457). May participate in the lipid metabolism (PubMed:9278457)..
Tissue specificity :Predominantly expressed in heart, skeletal muscle, and adrenal cortex. Also found in brain, placenta, liver, kidney, pancreas, thyroid, testis and ovary. Almost absent in lung, thymus and peripheral blood leukocytes..
Subcellular locationi :Endoplasmic reticulum membrane,Multi-pass membrane protein. Microsome membrane,Multi-pass membrane protein. Microsome membrane,Lipid-anchor. Membrane.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.