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Western blot(SDS PAGE) analysis of extracts from Daudi cells treated with 20 U/ml IFN alpha for 24 hours.Using MX1Rabbit mAb [H4CO] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MX1,MX1_HUMAN,Interferon-induced GTP-binding protein Mx1 [Cleaved into: Interferon-induced GTP-binding protein Mx1, N-terminally processed]
UniProtID :P20591
MASS(da) :75,520
MW(kDa) :76 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human MX1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected : Daudi cells treated with 20 U/ml IFN alpha for 24 hours
Function : Interferon-induced dynamin-like GTPase with antiviral activity against a wide range of RNA viruses and some DNA viruses. Its target viruses include negative-stranded RNA viruses and HBV through binding and inactivation of their ribonucleocapsid. May also antagonize reoviridae and asfarviridae replication. Inhibits thogoto virus (THOV) replication by preventing the nuclear import of viral nucleocapsids. Inhibits La Crosse virus (LACV) replication by sequestering viral nucleoprotein in perinuclear complexes, preventing genome amplification, budding, and egress. Inhibits influenza A virus (IAV) replication by decreasing or delaying NP synthesis and by blocking endocytic traffic of incoming virus particles. Enhances ER stress-mediated cell death after influenza virus infection. May regulate the calcium channel activity of TRPCs..
Subcellular locationi :Cytoplasm. Endoplasmic reticulum membrane,Peripheral membrane protein,Cytoplasmic side. Cytoplasm, perinuclear region.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.