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Western blot(SDS PAGE) analysis of extracts from U-87 MG cells.Using NRP2Rabbit mAb [77DK] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :NRP2,VEGF165R2,NRP2_HUMAN,Neuropilin-2
UniProtID :O60462
MASS(da) :104,831
MW(kDa) :130 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human NRP2
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :U-87 MG cells
Function : High affinity receptor for semaphorins 3C, 3F, VEGF-165 and VEGF-145 isoforms of VEGF, and the PLGF-2 isoform of PGF., (Microbial infection) Acts as a receptor for human cytomegalovirus pentamer-dependent entry in epithelial and endothelial cells..
Subcellular locationi :Membrane,Single-pass type I membrane protein.,[Isoform s9]: Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.