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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using FAM50A/XAP5Rabbit mAb [OT28] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :FAM50A,DXS9928E,HXC26,XAP5,FA50A_HUMAN,Protein FAM50A
UniProtID :Q14320
MASS(da) :40,242
MW(kDa) :40 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human FAM50A/XAP5
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Probably involved in the regulation of pre-mRNA splicing..
Tissue specificity :Widely expressed in fetal and adult tissues. Mostly abundant in fetal brain, liver and kidney,in the adult, high levels were also observed in heart, skeletal muscle, spleen, thymus, prostate and small intestine. Expressed in fetal cerebellum and hypothalamus. Low expression is observed in fetal temporal lobe (PubMed:32703943)..
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.