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Western blot(SDS PAGE) analysis of extracts from Mouse brain.Using IhhRabbit mAb [44I4] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :IHH,IHH_HUMAN,Indian hedgehog protein [Cleaved into: Indian hedgehog protein N-product,Indian hedgehog protein C-product]
UniProtID :Q14623
MASS(da) :45,251
MW(kDa) :45 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Ihh
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Mouse brain
Function : Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP) (By similarity)..
Tissue specificity :Expressed in embryonic lung, and in adult kidney and liver.
Subcellular locationi :[Indian hedgehog protein N-product]: Cell membrane,Lipid-anchor,Extracellular side.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.