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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using NHP2L1Rabbit mAb [V5FL] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SNU13,NHP2L1,NH2L1_HUMAN,NHP2-like protein 1 [Cleaved into: NHP2-like protein 1, N-terminally processed]
UniProtID :P55769
MASS(da) :14,174
MW(kDa) :14 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human NHP2L1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Involved in pre-mRNA splicing as component of the spliceosome (PubMed:28781166). Binds to the 5'-stem-loop of U4 snRNA and thereby contributes to spliceosome assembly (PubMed:10545122, PubMed:17412961). The protein undergoes a conformational change upon RNA-binding (PubMed:17412961)..
Tissue specificity :Ubiquitous.
Subcellular locationi :Nucleus. Nucleus, nucleolus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.